Method for assessing the frequency of transfer of an R factor on solid media and for isolating cells of the donor type from a predominantly repressed culture.

نویسندگان

  • P A Lee
  • M H Richmond
چکیده

A method for assessing the incidence of R factor transfer on solid media is described. The method gives values similar to those obtained with conventional techniques but allows the derepressed cells from a predominantly repressed population to be isolated.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and optimization of mice skeletal muscle satellite cells using preplating method and culture media substitution

Introduction: Satellite cells are known as the main regenerative cell type in skeletal muscles. Our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. Low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. M...

متن کامل

The effect of Fibroblast Growth Factor-2(FGF-2) and retinoic acid on differentiation of mouse embryonic stem cells into neural cells

Introduction: Embryonic Stem (ES) cells as pluripotent cells derived from the inner cell mass of blastula can differentiate to neural cells in vitro and this property is valuable in studies of neurogenesis and in the generation of donor cells for transplantation. In this regard, the propose of this research, was the study of the role of two important factors in the development of neural syst...

متن کامل

Probable Limitations of Osteoblasts Isolation from the Periosteum and Alveolar Bone in a Dog Model

Background & Objective: Bone defects in jaws create major problems for oral and maxillofacial surgery. To overcome the limitations of Autografts tissue engineering uses autogenus cells and synthetic scaffolds. Type of cells or cell sources have an important effect on the construction which is produced. The aim of this study was to evaluate the feasibility and probable limitations of osteoblast...

متن کامل

Efficiency of Ovine Fibroblast or Cumulus Cells For Somatic Cell Nuclear Transfer in Sheep

Purpose: Despite remarkable progresses have been achieved in the field of somatic cell nuclear transfer (SCNT), there is little information regarding the effect of donor cell type on the efficiency mammalian somatic cell cloning in vitro. This study compared in vitro developmental competency of sheep enucleated oocytes reconstructed with either fibroblast or cumulus cells. Material and methods...

متن کامل

Using Lattice Boltzmann Method to Investigate the Effects of Porous Media on Heat Transfer from Solid Block inside a Channel

A numerical investigation of forced convection in a channel with hot solid block inside a square porous block mounted on a bottom wall was carried out. The lattice Boltzmann method was applied for numerical simulations. The fluid flow in the porous media was simulated by Brinkman-Forchheimer model. The effects of parameters such as porosity and thermal conductivity ratio over flow pattern and t...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 100 2  شماره 

صفحات  -

تاریخ انتشار 1969